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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
26/12/2003 |
Data da última atualização: |
26/12/2003 |
Autoria: |
ORMOND, J. G. P.; PAULA, S. R. L. de; FAVERET FILHO, P.; ROCHA, L. T. M. da. |
Título: |
Agricultura orgânica: quando o passado é futuro. |
Ano de publicação: |
2002 |
Fonte/Imprenta: |
Revista de Política Agrícola, Brasília, ano 11, nº 1, p. 57-74, Jan./Fev./Mar. 2002. |
Idioma: |
Português |
Categoria do assunto: |
-- |
Marc: |
LEADER 00452naa a2200145 a 4500 001 1109251 005 2003-12-26 008 2002 bl uuuu u00u1 u #d 100 1 $aORMOND, J. G. P. 245 $aAgricultura orgânica$bquando o passado é futuro. 260 $c2002 700 1 $aPAULA, S. R. L. de 700 1 $aFAVERET FILHO, P. 700 1 $aROCHA, L. T. M. da 773 $tRevista de Política Agrícola, Brasília, ano 11, nº 1, p. 57-74, Jan./Fev./Mar. 2002.
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Embrapa Unidades Centrais (AI-SEDE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
07/12/2012 |
Data da última atualização: |
22/01/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
LOBO, A. M. B. O.; GUIMARÃES, S. E. F.; PAIVA, S. R.; CARDOSO, F. F.; SILVA, F. F.; FERNANDES JÚNIOR, G. A.; LOBO, R. N. B. |
Afiliação: |
ANA MARIA BEZERRA OLIVEIRA LOBO, CNPC; SIMONE ELIZA FACCIONI GUIMARÃES, UFV; SAMUEL REZENDE PAIVA, CENARGEN; FERNANDO FLORES CARDOSO, CPPSUL; FABYANO FONSECA SILVA, UFV; GERARDO ALVES FERNANDES JÚNIOR, UNESP; RAIMUNDO NONATO BRAGA LOBO, CNPC. |
Título: |
Differentially transcribed genes in skeletal muscle of lambs |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Livestock Science, Amsterdam, v. 150, n. 1/3, p. 31-41, Dec. 2012. |
Idioma: |
Inglês Português |
Conteúdo: |
Abstract: The objective of this study was to compare gene transcription profiles in Longissimus dorsi muscle of the following four hair sheep genetic groups, Morada Nova (MO), Brazilian Somali (SO), Santa Inês (SI) and ½Dorper×½Morada Nova (F1). These groups all display different postnatal muscle growth. The transcriptomes of the skeletal muscle of the lambs (at 200 days of age) were profiled by using oligonucleotide microarrays and reverse transcription-quantitative real-time PCR (RT-qPCR). The microarray experiment identified 262 transcripts that were differentially expressed when transcription levels were compared between the different breeds. A total of 23 transcripts among which those involved in skeletal muscle development (MyoD1 and IGFBP4), lipogenesis and adipogenesis (C/EBP?, PPAR? and PGDS) were differentially expressed in at least in one comparison. Clustering analysis showed that there is greater similarity in gene expression between the MO and SI breeds and between F1 and SO genetic groups. The SO breed has the most distinct expression pattern. The RT-qPCR results confirmed the findings from the microarray study. A positive correlation was observed between the expression of MyoD1 and the cold carcass yield. The negative correlations between the weight and yield of cold carcass with the expression of C/EBP? mean that the selection for adipogenesis could lead to a lower carcass weight. The GLUT3 and PYGL gene transcripts were negatively correlated with fat thickness, but ATP5G1 was positively correlated with this trait. Interestingly, many genes negatively correlated with PUFA were positively correlated with cold carcass yield. In conclusion, the present work demonstrated that there are breed-specific expression patterns in Brazilian hair sheep genetic groups. The differences in gene expression among genetic groups were consistent with their phenotypic differences. The positive correlation of the MyoD1 expression with the cold carcass yield suggests that this gene is important for tissue growth in sheep. The positive correlation of the C/EBP? expression with PUFA provides an opportunity to select for lipid deposition in meat animals. MenosAbstract: The objective of this study was to compare gene transcription profiles in Longissimus dorsi muscle of the following four hair sheep genetic groups, Morada Nova (MO), Brazilian Somali (SO), Santa Inês (SI) and ½Dorper×½Morada Nova (F1). These groups all display different postnatal muscle growth. The transcriptomes of the skeletal muscle of the lambs (at 200 days of age) were profiled by using oligonucleotide microarrays and reverse transcription-quantitative real-time PCR (RT-qPCR). The microarray experiment identified 262 transcripts that were differentially expressed when transcription levels were compared between the different breeds. A total of 23 transcripts among which those involved in skeletal muscle development (MyoD1 and IGFBP4), lipogenesis and adipogenesis (C/EBP?, PPAR? and PGDS) were differentially expressed in at least in one comparison. Clustering analysis showed that there is greater similarity in gene expression between the MO and SI breeds and between F1 and SO genetic groups. The SO breed has the most distinct expression pattern. The RT-qPCR results confirmed the findings from the microarray study. A positive correlation was observed between the expression of MyoD1 and the cold carcass yield. The negative correlations between the weight and yield of cold carcass with the expression of C/EBP? mean that the selection for adipogenesis could lead to a lower carcass weight. The GLUT3 and PYGL gene transcripts were negatively correlated with fat thickn... Mostrar Tudo |
Palavras-Chave: |
Cluster; Cluster sampling; Differential expression; IGFBP4; MyoD1. |
Thesagro: |
Genética animal; Ovino; Ovis Aries. |
Thesaurus NAL: |
Animal genetics; cluster analysis; Gene expression; Sheep. |
Categoria do assunto: |
-- G Melhoramento Genético |
Marc: |
LEADER 03105naa a2200337 a 4500 001 2034773 005 2016-01-22 008 2012 bl uuuu u00u1 u #d 100 1 $aLOBO, A. M. B. O. 245 $aDifferentially transcribed genes in skeletal muscle of lambs$h[electronic resource] 260 $c2012 520 $aAbstract: The objective of this study was to compare gene transcription profiles in Longissimus dorsi muscle of the following four hair sheep genetic groups, Morada Nova (MO), Brazilian Somali (SO), Santa Inês (SI) and ½Dorper×½Morada Nova (F1). These groups all display different postnatal muscle growth. The transcriptomes of the skeletal muscle of the lambs (at 200 days of age) were profiled by using oligonucleotide microarrays and reverse transcription-quantitative real-time PCR (RT-qPCR). The microarray experiment identified 262 transcripts that were differentially expressed when transcription levels were compared between the different breeds. A total of 23 transcripts among which those involved in skeletal muscle development (MyoD1 and IGFBP4), lipogenesis and adipogenesis (C/EBP?, PPAR? and PGDS) were differentially expressed in at least in one comparison. Clustering analysis showed that there is greater similarity in gene expression between the MO and SI breeds and between F1 and SO genetic groups. The SO breed has the most distinct expression pattern. The RT-qPCR results confirmed the findings from the microarray study. A positive correlation was observed between the expression of MyoD1 and the cold carcass yield. The negative correlations between the weight and yield of cold carcass with the expression of C/EBP? mean that the selection for adipogenesis could lead to a lower carcass weight. The GLUT3 and PYGL gene transcripts were negatively correlated with fat thickness, but ATP5G1 was positively correlated with this trait. Interestingly, many genes negatively correlated with PUFA were positively correlated with cold carcass yield. In conclusion, the present work demonstrated that there are breed-specific expression patterns in Brazilian hair sheep genetic groups. The differences in gene expression among genetic groups were consistent with their phenotypic differences. The positive correlation of the MyoD1 expression with the cold carcass yield suggests that this gene is important for tissue growth in sheep. The positive correlation of the C/EBP? expression with PUFA provides an opportunity to select for lipid deposition in meat animals. 650 $aAnimal genetics 650 $acluster analysis 650 $aGene expression 650 $aSheep 650 $aGenética animal 650 $aOvino 650 $aOvis Aries 653 $aCluster 653 $aCluster sampling 653 $aDifferential expression 653 $aIGFBP4 653 $aMyoD1 700 1 $aGUIMARÃES, S. E. F. 700 1 $aPAIVA, S. R. 700 1 $aCARDOSO, F. F. 700 1 $aSILVA, F. F. 700 1 $aFERNANDES JÚNIOR, G. A. 700 1 $aLOBO, R. N. B. 773 $tLivestock Science, Amsterdam$gv. 150, n. 1/3, p. 31-41, Dec. 2012.
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